When I chord with the note, I get no out of tune beating. Happens in different rooms, and only on that B note, especially with the Blues Jr. Thought it might be a resonant tube, but no deal. Other guitars are fine through those two amps, in the studio or on the gig. Yet, a tuner always confirms that it's dead on. The B note on the low E of my old Tele sometimes sound 1/2 step sharp through my Blues Jr and my 64 Vibroverb, but not through any other amp. It's a bear when you're trying to sing at a gig. I've had this happen to me with certain situations on the gig and in the studio. Just hoping for some insight and suggestions. Maybe I should remove it from the chain and see what happens. Lately I have been recording through a pre (nothing fancy, just a lowly ART Tube MP studio V3). I don't know if its related to the fact that my room is essentially untreated (for now, soon to be rectified), is it inherent to my system somehow, or am I going nuts or even deaf (I doubt the latter, I have always worn hearing protection when needed). Now I seem to notice it on everything I listen through. I noticed the problem in the past when listening on my cans but always attributed this to the lack of low range on the headphones.
For referencing, I use Sonodyne SM 100 Ak monitors and I will flip over to a surround sound system (THX certified Atlantic Technology system with a big sub) on the other side of this non-dedicated room for a second listen to my mix. All other F#'s sound fine, but of course they are all 1 or more octaves higher. when tuned correctly, the strobe confirms the suspect note is in tune.
I can make it a little sharp or flat with no benefit, and of course that throws the rest of the instrument off. My bass (and all my other stringed instruments) is properly intonated, it has new strings, the frets are in perfect shape it is tuned correctly as per my ear and a strobe tuner, yet while recording, the darn low F# never sounds in tune.